Spectrophotometric estimation of cefixime and ofloxacin from tablet dosage form

Abstract

Two simple, rapid, accurate and precise spectrophotometric methods have been developed for simultaneous estimation of Cefixime and Ofloxacin from tablet dosage form. Method І involves formation of ‘simultaneous equations’ at 234 nm (λ max of Cefixime) and 296 nm (λ max of Ofloxacin); while Method ІІ involves, formation of ‘Absorbance ratio equation’ at 275(isoabsorptive point) and 296 nm (lmax of Ofloxacin) using methanol as a solvent. The linearity was observed in the concentration range of 4 - 20 µg/ml for Cefixime and 2 - 10 µg/ml for Ofloxacin. The results of analysis have been validated statistically and by recovery studies and were found satisfactory.