Simultaneous estimation of moxifloxacin HCl and prednisolone acetate from eye drop formulation

Abstract

A simple, fast, and precise simultaneous spectrometric method for the estimation of Moxifloxacin HCl and Prednisolone acetate in API and in Formulation was developed. The proposed method is based on the formation and solving of simultaneous equations using 288 nm and 247 nm as two analytical wavelengths. Moxifloxacin HCl shows absortion maximum at 288 nm and Prednisolone acetate shows absorbance at 247 nm in water (co-solvent Methanol). Beer’s law was obeyed in the concentration range of 2- 12μg/ml for Moxifloxacin HCl and 2-12 μg/ml for Prednisolone acetate. The method allows rapid analysis of binary pharmaceutical formulation with accuracy. The results of analysis have been validated statistically and recovery studies confirmed the accuracy of the proposed method. The developed method was found to be very precise as % C.V calculated came out to be less than 2%.