Newer RP-HPLC method development and validation of cefixime and linezolide in bulk drugs and combined dosage form

Abstract

Newer RP-HPLC method was developed for simultaneous determination of cefixime (CEFI) and linezolide (LINZ) in bulk drugs and combined dosage form. Mobile phase consisting of mixture of methanol and water (pH 3.2 adjusted with 1% O-phosphoric acid) in the ratio 40: 60 at flow rate of 1.0 ml/min using C18 Grace (250mmX 4.6mm) column at 252 nm. The retention time of CEFI and LINZ was found to be 4.81 min and 7.16 min, respectively. The linearity range for CEFI with LINZ observed was 1-5 μg/ml and 3-15 μg/ml, respectively. Method was validated as per ICH guidelines. Validation parameters studied were linearity and range, recovery study, precision, LOD, LOQ and robustness. Statistical data obtained was found to satisfactorily.