Validated UV-Spectrophotometric methods for Simultaneous Estimation of Rabeprazole and Ondansetron in Bulk drug and Tablet formulation

Abstract

Three simple, accurate and precise spectrophotometric methods have
been developed for simultaneous determination of Rabeprazole (RAB)
and Ondansetron (OND) in a laboratory mixture. Simultaneous equation
method (Method I) shows absorbance at 284.5 nm (λ1) and 307 nm (λ2)
corresponding to the absorbance maxima of RAB and OND respectively.
In absorbance ratio method (Method II) isobestic point is observed at 291
nm. Isobestic point 291 nm is considered as (λ1) and absorbance maxima
of OND at 307 nm are considered as (λ2). In First order derivative zero
crossing method (Method III) (λ1) 247nm (for RAB) and (λ2) 320.5 nm
(for OND) was carried out. All dilutions were prepared in distilled water
and methanol (50:50). Linearity range was observed in the concentration
range of solution 2.5‐ 12.5 μg/ml for RAB and 4-12 μg/ml for OND. The
methods were validated statistically and recovery study was performed to
confirm the accuracy of both drugs. The developed methods are simple,
economic, accurate, precise and reproducible. They can be adopted for
routine quality control analysis of these drugs in pharmaceutical
combined dosage form.