Cyclophosphamide-induced oxidative stress in brain: Protective effect of Garcinia indica fruit extract

Abstract

This study sought to characterize the distribution of phenols and antioxidant activities in Garcinia Indica fruit extract and their inhibition of cyclophosphamide-induced oxidative stress in rat’s brain. The total Phenol content and antioxidant activities of Garcinia Indica fruit extract were determined in vitro and in vivo. The Results of the study revealed that intraperitoneal administration of cyclophosphamide (75mg/kg of body weight) caused a Significant increase (P<0.05) in the malondialdehyde (MDA) content of the brain; however, there was a significant Decrease (P<0.05) in the brain MDA content, in those of rats treated with Garcinia Indica fruit extract showed a higher Inhibitory effect. In addition, treatment of the Garcinia Indica fruit extract also caused a dose-dependent inhibition of Serum glutamate oxaloacetate transaminase (SGOT), Serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase and total bilirubin; likewise, treatment of the Garcinia Indica fruit extract inhibited MDA production. The higher Inhibition of oxidative stress in brain and serum enzymes and metabolites by the Garcinia Indica fruit extract could be attributed to its significantly higher (P<0.05) total phenol content, reducing power and free-radical scavenging ability. Therefore, treatment of Garcinia Indica fruit extract could prevent cyclophosphamide-induced oxidative stress in brain; although the Garcinia Indica fruit extract is a better protectant.