In -Vitro antioxidative activity of phenolic and flavonoid compounds extracted from fruit of Garcinia indica

Abstract

The aim of this work was to estimate the total phenolic and flavonoid content, and to evaluate in-vitro antioxidant activity of Methanolic fruit extract of Garcinia Indica. The raw, dry fruit powder was extracted with 99.9% of methanol. Phytochemical test shows that extract contains higher level of total phenol and flavonoids. Total phenolic compound in methanolic fruit extract of Garcinia Indica was found to be 0.348 mg/g of extract calculated as gallic acid equivalent (R² = 0.985) and total flavonoids compound was found to be 137.27 µg/g of extract calculated as quercetin equivalent (r2=0.997). The extract was screened for its potential antioxidant activities using tests such as hydroxyl radical-scavenging activity, reducing power activity, and hydrogen peroxide-scavenging activity. The in-vitro antioxidant assay showed Garcinia Indica posses potent antioxidant activity when compared with reference compound ascorbic acid. Garcinia Indica could be useful for preparation of neutraceuticals as potent antioxidant to treat various human diseases and its complications.