The In Vitro antioxidant and free radical scavenging Activities of suran (Amorphophallus campanulatus (Araceae)) tubers extracts

Abstract

Total Phenolics and the antioxidative properties of Amorphophallus campanulatus were studied. The dried Powder of tubers was extracted successively with methanol, Hydroalcohol, ethanol and 70% acetone separately. After removing the solvents, the extracts were freeze-dried. The Amorphophallus campanulatus tubers contained relatively high levels of total Phenolics and tannins. The extracts were subjected to assess their potential antioxidant activities using systems such as diphenyl- β-picrylhydrazyl (DPPH˙), ABTS͘⁺, FRAP, linoleic acid emulsion, O₂⁻and OH͘´. The superoxide anion radical-scavenging activity was found to be higher in methanolic extract in comparison to other extracts’ at the concentration of 600 µg in the reaction mixture. The DPPH radical and ABTS action radical-scavenging activities were well proved and related with the ferric-reducing/antioxidant capacity of the extracts. Interestingly, among the various extracts, methanolic extracts’ shows significantly (P < 0.05) higher hydroxyl radical- scavenging activity. In general, all extracts exhibited good antioxidant activity (53.3–73.1%) against the linoleic acid emulsion system but were significantly (P < 0.05) lower than the synthetic antioxidant, BHA (93.3%).