Potential activity of aqueous extract of Corn Silk onCarrageenan induced anti-inflammatory and antioxidant activity Using Albino Rats

Abstract

In the present study an attempt has been made to find out Potential activity of aqueous extract of Corn Silk on Carrageenan induced anti-inflammatory and antioxidant activity Using Albino Rats. Corn silk has a great medicinal importance due the presence of a variety of bioactive phytochemical compounds. The principal phytochemicals present in corn silk include polyphenols, phenolic acids, flavonoids, anthocyanins, glycosides, carotenoids, and some water-soluble vitamins. Active ingredients contributing to anti-inflammatory property are tannins, phenols, alkaloids, flavonoids, carotenoids, β-sitosterol, vanillin, hydroxymellein, moringine, moringinine, β-sitostenone, and 9-octadecenoic acid. The aquaous extract of corn silk at doses 100 and 200 mg/kg produced a significant effect against carrageenan induced inflammatory effect and the results were comparable to that of control. Anti- inflammatory activity of aquaous extract of corn silk showed significant and similar to that of indomethacine (10 mg/kg). It was observed that the aquaous extract of corn silk (400 mg/kg, p.o.) exhibits maximum anti- inflammatory activity against Carrageenan induced hind paw edema. Nitric oxide scavenging activity was measured by using UV-Visible spectrophotometer. Sodium nitroprusside (5mM) in phosphate buffer was mixed with different concentrations of EEET (25-800 μg/ml), dissolved in normal saline and incubated at 25°C for 30 min. Control without test compound but with equivalent amount of sodium nitroprusside was taken. Aquaous extract of corn silk known to scavenge the free radicals generated by ABTS by donating a hydrogen atom indicating aquaous extract of corn silk a potent anti-oxidant. Decolorization of ABTS is observed which expressed IC₅₀value of 60μg/ml.