Identification of steroid compound using preparative Thin Layer Chromatography, GC-MS and antimicrobial and antioxidant properties of Cenchrus setigerus (Poaceae)

Abstract

The present investigation was carried out to determine the possible bioactive components of Cenchrus setigerus using PTLC, IR spectra and GC-MS analysis, in vitro antibacterial activity of methanolic extracts and comparative estimation of antioxidant properties (in-vitro and in-vivo) in terms of POX, CAT, PPO, SOD, LPO activity and carotenoids, total phenolics contents of C. setigerus (Poaceae) were done. Antibacterial activity was evaluated against seven Gram-negative bacteria, two Gram-positive bacteria and three fungi, using disk diffusion method followed by determination of minimum inhibitory concentrations (MIC) by broth dilution method, against sensitive bacteria and fungi. Result reveals that the most bioactive compound in C. setigerus was stigmasterol. The highest activity was exhibited by the root extract against Bacillus subtilis. Klebsiella pnemoniae, Proteus merabilis and Aspergillus flavus were the most sensitive pathogens witch show maximum antimicrobial effects. Highest antioxidant properties of C. setigerus were found to greater in in-vitro then in-vivo.