Antimicrobial activity of ethanolic, methanolic and hot aqueous extract of Punica granatum peel

Abstract

The main objective of the study is to evaluate antibacterial activity of Punica granatum  pericarp (peel) extracts (hot aqueous, methanolic and ethanolic) against E. coli and P. aeruginosa, using Zone of Inhibition (ZOI) and Minimum Inhibitory Concentration (MIC) method. Hot aqueous, methanolic and ethanolic extracts of P. granatum pericap showed an average inhibitory zone diameter of 21.5, 22.5 and 23mm respectively which indicates that ethanolic extract showed best result having ZOI greater than that of the standard antibiotic Tetracycline (20.1mm) against E. coli while  hot aqueous, methanolic and ethanolic extracts of P. granatum pericap showed an average inhibitory zone diameter of 21, 23.5 and 25.5mm respectively which indicates that ethanolic extract showed best result having ZOI greater than that of the standard antibiotic Tetracycline (22.5 mm) against P. aeruginosa. P. granatum peel has MIC of 0.312 µg/ml against E. coli and 0.421 µg/ml against P. aeruginosa. Data revealed that ethanolic extract of P. granatum peel has shown highest antimicrobial activity as compared to other extracts.