MIC, MBC AND MFC activity of diffreent extracts of Terminalia belerica

Abstract

The present study was done to analyze the antimicrobial activity of different extracts of Terminalia belerica. Medicinal plants serve as potent antimicrobial agents. Here, the ethanolic, methanolic, choloroform, ethyl acetate and aqueous extracts of Terminalia belerica were examined for their antibacterial activity against gram positive and gram negative bacterial strains using disk diffusion technique. Further, Minimum inhibitory concentrations (MICs) of the extracts were also determined using microbroth dilution test. It was observed that all the extracts of Terminalia belerica possess high antimicrobial potential against maximum test bacteria used. Maximum zone of inhibition was observed for chloroform extract of Terminalia belerica against Enterococcus faecalis ATCC 29212 (29 mm) with MIC 10.4 µg/ml and MLC 41.7 µg/ml among gram positive bacteria. Whereas, among gram negative bacteria maximum zone of inhibition was observed in case of methanolic extract against Escherichia coli ATCC 11840 (20 mm) with MIC 20.8 µg/ml and MLC 83.3 µg/ml.