A study on isolation of different type of bacteria from pus

Abstract

The microbiological analysis of infection in 245 patients was undertaken in the outpatient departments of the Pt. Jawahar lal Nehru memorial medical college and Dr. B.R.A.M. hospital, Raipur (C.G.). Identification of bacterial isolates was determined by standard microbiological techniques. A total of one hundred and sixteen bacterial isolates were obtained from different cultures. In 86 cases, cultures were monomicrobial, 16 cultures were polymicrobial but no bacterial isolate was obtained in 149 cases. Staphylococcus aureus was the predominant microorganism (40%) followed by Klebsiella sp. (33%), Pseudomonas sp. (18%), Escherichia coli (16%), and Proteus sp. (7%). The diversity of microorganisms and the high incidence of polymicrobic flora in this study give credence to the value of identifying one or more bacterial pathogens from pus cultures. Continuous dialogue between the microbiology department and wound care practitioners and education of patients on personal hygiene is strongly advised.