Preliminary Phytochemical, Physicochemical and Quantitative Evaluation of Piper nigrum Fruit

Abstract

This study focuses on the preliminary phytochemical, physicochemical, and quantitative evaluation of Piper nigrum fruit. Various extraction and analysis techniques were employed to determine the plant's chemical composition, physicochemical parameters, and active constituent content. Results revealed the presence of key phytochemicals like alkaloids, flavonoids, tannins, and volatile oils. Physicochemical parameters, including moisture content, ash values, and extractive values, were found within pharmacopoeial limits. The active constituent piperine was quantified using HPLC and TLC methods, demonstrating the efficacy of both laboratory-prepared and marketed extracts. The study provides a foundational understanding of the bioactive compounds in Piper nigrum and its potential therapeutic applications.