Simultaneous estimation of finasteride and tamsulosin hydrochloride by reverse phase HPLC in bulk and pharmaceutical dosage form

Abstract

This investigation describes a new simple, precise, sensitive and accurate RP-HPLC method for the Simultaneous estimation of Finasteride and Tamsulosin Hydrochloride in Bulk and Tablets. The resolution of two drugs was achieved on Symmetry C₁₈ (150mm x 4.6mm i.d., 5mm particle size) column with UV detection at 245 nm and the mobile phase consist of Buffer and Acetonitrile and Water (15:75:10v/v).Using chromatographic conditions described Finasteride and Tamsulosin Hydrochloride were well resolved with mean retention times of 2.325 and 4.296 min, respectively. Linear response (r>0.999) was observed over the range of 62-312µg/mL for Finasteride and 5-25µg/mL for Tamsulosin Hydrochloride. The lower limit of quantification and lower limit of detection was 9.95 and 2.95 for Finasteride and 9.91and 2.97 for Tamsulosin Hydrochloride. The Validation parameters were performed according to the ICH guidelines and the proposed method can be useful in the routine analysis for the determination of Finasteride and Tamsulosin Hydrochloride in Pharmaceutical dosage forms.