RP-HPLC Method Development, Validation and Stability Indicating Study of Olanzapine in Tablet Dosage Form

Abstract

RP-HPLC method was developed for evaluation of Olanzapine in
pharmaceutical formulation. The separation was conducted by using
mobile phase consisting of buffer (KH2PO4): acetonitrile in the ratio of
60:40.The wavelength was found at 257.0nm and it is freely soluble in
0.1 NHCL, methanol, acetonitrile and benzene and slightly soluble in
ethyl alcohol. The linearity of method was investigated in the range of 5-
25 µg/ml and R.S.D was found 1.994. The aim of this research study was
to develop and validate simple, accurate, precise, sensitive and cost
effective RP-HPLC method for quantitative evaluation of Olanzapine
drugs and to develop a validated Stability Indicating RP-HPLC method
for determination of Olanzapine drug in pharmaceutical formulations
which are critical for the quality control laboratories.