TD-DAMD and TU-DAMD polymorphism in Arthrocnemum macrostachyum Moris species

Abstract

Arthrocnemum macrostachyum (Moric.) Moris. & Delponte perennial succulent
halophyte shrub suffered from taxonomic confusion. Thereby, the present study
focused on phylogenetic relationships investigation among three A.
macrostachyum genotypes grown along the Syrian coast. DNA polymorphism
of A. macrostachyum has been investigated based on touch-down directed
amplification of minisatellite DNA (Td-DAMD) and touch up-directed
amplification of minisatellite DNA (TU-DAMD) markers in comparative study.
Seventeen DAMD primers were tested for each TD-DAMD and TU-DAMD
assesses. Comparative study revealed that TU-DAMD assay was more potent
than TD-DAMD one by exhibiting the highest polymorphism level (P%)
92.035% & 87.805% of and polymorphic information content (PIC) values of
and 0.411 and 0.386, for TU-DAMD and TD-DAMD, respectively. Clustering
profile constructed based on the both examined assesses gave similar pattern;
where, A. macrostachyum2 genotype was genetically so far from the other two
genotypes. Thereby, the actual data confirmed previous reports suggesting the
existence of two subspecies belonged to A. macrostachyum species. Otherwise,
Due to efficacy of TU-DAMD for genetic variation revealing of A.
macrostachyum species; it is advice to employ this technique as a new assay in
phylogenetic studies of other plant species.