In vitro evaluation of Aldose reductase inhibitory potential of Bougainvillea spectabilis

Abstract

The dried Leaves powder of Bougainvillea spectabilis Linn was extracted by successive solvent extraction method
to obtained hydroalcholic and aqueous extract. Water saturated n-butanol was used for further extraction of the dried
hydroalcoholic extract. The layers were separated and n-butanol layer was acidified with 1 N KOH to obtain the raw
saponin extract. All the extracts were screened for invitro aldose reductase inhibitory activity in purified goat lens
using Hayman and Kinoshita method in which decrease in NADPH concentration was estimated at 340nm using UV
Visible spectrophotometer. From the result it was observed that all the three extracts inhibit AR activity, but at
different extent. From dose response curve it was found that saponin extract (SE) is more effective followed by
aqueous extract (AE) and methanolic extract (ME) with IC50 values of 32.66 ±0.33 µg/ml , 73.48 ±1.13 µg/ml and
131.0 ±1.65 µg/ml respectively. In the end it was concluded that among the three extracts, saponin extract of
Bougainvillea spectabilis is potent in inhibiting the aldose reductase enzyme which contribute major role in the
diabetes complication.