Phytochemical Screening and HPTLC Fingerprinting Profile of bark extracts of Ehretia laevis

Abstract

To establish the Preliminary Phytochemical Screening and fingerprint profile of bark Extracts of Ehretia laevis by using high performance thin layer chromatography (HPTLC) technique. Preliminary phytochemical screening of extracts were done, physical constants were evaluated and HPTLC studies were carried out. CAMAG make HPTLC system equipped with Linomat 5 applicator, TLC scanner 3, server vision CATS-server PH, version 2.5.18072.1 software were used. Preliminary phytochemical screening of the extract showed the presence of Flavonoids, glycosides, tannins, alkaloids, terpenoids, phenolic compounds, proteins, reducing sugars, fats and oils. The fingerprint analysis of petroleum ether extract showed four peaks, chloroform extract showed four peaks and methanolic extract showed six peaks in 10 μl of the sample analysed. It can be concluded that HPTLC fingerprint analysis of Petroleum ether, chloroform and methanolic extract of bark powder of Ehretia laevis can be used as a diagnostic tool for the correct identification of the plant and it is useful as a phytochemical marker and also a good estimator of genetic variability in plant populations.