HPLC Method validation for estimation of Atenolol and Indapamide in tablet dosage form

Abstract

The present High Performance liquid chromatographic method is to determine Atenolol and Indapamide from its formulation. Various experiments were carried out to establish the method. The mobile phase was methanol : water (55:45) with 0.1% v/v of Ammonium Hydroxide is found to be ideal for the estimation of Atenolol and Indapamide. The elution order was as followed (Atenolol – 7.5min, Indapamide – 8.9 min). The values of linearity, Precision and standard deviation show that the proposed method was reproducible, accurate and precise.