Sensitive estimation of olmesartan medoxomil tablets by RP-HPLC method

Abstract

A simple, selective, rapid, precise and economical reverse phase high pressure liquid chromatographic method has been developed for the estimation of Olmesartan medoxomil from pharmaceutical Tablet dosage form. The method was carried out on a C₁₈ (250 mm x 4.6 mm i.d., 5 µ) column with a mobile phase consisting of acetonitrile: 5 mM ammonium acetate (adjusted to pH 4.5 using orthophosphoric acid) (60:40 v/v) at a flow rate of 1.0 ml/min. Detection was carried out at 255 nm. The retention time of Olmesartan medoxomil was 4.9 min. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantitation and solution stability.The proposed method can be used for the estimation of these drugs in dosage forms.