Simultaneous estimation of atenolol, hydrochlorothiazide, losartan and valsartan in the pharmaceutical dosage form
Keywords:
Validation, RP-HPLC, Atenolol (ATEN), Hydrochlorothiazide (HYD), Losartan (LOS) and Valsartan (VAL).Abstract
A simple, specific, accurate and economical isocratic reversed phase liquid chromatographic (RP-HPLC) method was developed and subsequently validated for the determination of atenolol, hydrochlorothiazide, losartan and valsartan. Separation was achieved with a Nucleodur 100 C–18 column having 250 x 4.6mm i.d. with 5μm particle size and potassium dihydrogen phosphate buffer adjusted to pH 3.0 using diluted ortho phosphoric acid and acetonitrile (50:50 v ∕ v) with isocratic program as eluent at a constant flow rate of 1.0ml per min. UV detection was performed at 210nm. The retention time of atenolol, hydrochlorothiazide, losartan and valsartan was about 1.99min, 2.90min, 5.92min and 9.42min respectively. The proposed method was validated and successfully used for estimation of atenolol, hydrochlorothiazide, losartan and valsartan in the pharmaceutical dosage form.
