Development of medical textile product using chitosan incorporated herbal extract (Aristolochia bracteolate)

Abstract

In the current research, biopolymer herbal composites have been prepared using Chitosan and herbal extract of
Aristolochia bracteolate. It has wide range of application in the field of pharmaceutical industries. Chitosan was
isolated from exoskeleton of crab’s shell by means of demineralization and deacetylation process. Purification of
chitosan was achieved by NaOH and acetic acid. The herbal extract was prepared by 80% methanol. The composite
was prepared by mixing the biopolymer and herbal extract in a magnetic stirrer for 20 minutes. The prepared
biopolymer and the herbal extract composite were tested for its wound healing activity by using In vitro Wound
Scratch Assay. The wound healing study was performed in fibroblast cell lines at various concentrations. The
Chitosan incorporated herbal extract showed 80% wound healing activity in 75µL at 18 hrs time period. The
selected concentration was used for finishing the prepared biopolymer composite to the cotton woven fabrics. The
finished fabric was kept as a medicated area and were developed in to a medical product i.e. Band-Aid.