An Imperical study of Flavonoid Fraction of Juglans regia in Diabetic rats for their Wound Healing Activity

Abstract

The present work is deals with study of the wound healing activity of Flavonoid Fraction of Juglans regia Linn. (FFJR) in Diabetic Rats. From the fruits, the flavonoid fraction was prepared and flavonoid and phenolic compounds were determined. The diabetes was induced by the single administration of Streptozotocin in citrate buffer. The wound healing activity of flavonoid fraction was determined by Dead space, Incision and Excision wound models.  During the experimental period the fasting blood glucose level in diabetic animals were higher than those of normal animals. However, the fasting blood glucose level was significantly reduced in treatment groups. Topical application of flavonoid fraction, ointment base and Povidone iodine on dorsal inter scapular region causes significant and faster rate of wound closure and reduced epithelialization period.

In incision wound model, there is a significant increase in skin tensile strength by 10% w/w and 20% w/w ointment of FFJR compared to control. The hydroxyproline content was increased significantly in dose dependent manner by 20% FFJR. FFJR significantly decreased the epithelialization period, hence have significant effect on the proliferative phase of wound healing in diabetic rats.