Validation of UV-Spectrophotometric and RP-HPLC methods for the simultaneous analysis of Paracetamol and Aceclofenac in marketed tablets

Abstract

The aim of the study was to develop UV-spectrophotometric and RP-HPLC methods for the simultaneous analysis of paracetamol and aceclofenac in marketed tablets. The methods were validated in terms of linearity, accuracy (% Recovery), precision (inter day, intraday and reproducibility) and robustness. Both the methods were linear (R² = 0.997-0.999 for UV method and 0.999 for RP-HPLC method) and accurate (% recovery was 99.19% - 100.14%). The method was also found precise (% RSD< 2%) and robust. Potency of three marketed brands was determined by both the methods and no statistically significant difference was noticed between the potency obtained by two methods by paired t test at 5% significance level. Paracetamol released from marketed products was found to comply compendia specification but inter brand variation in case of aceclofenac release was observed by ANOVA (Sig. 0.000). Any one of the validated methods can be used for the analysis of paracetamol and aceclofenac tablets.