Estimation of nifedipine by reverse phase high performance liquid chromatography tablet dosage form

Abstract

A simple, selective, rapid, precise, sensitive and accurate HPLC method has been developed for the estimation of nifedipine using methanol and water (70:30 v/v) as mobile phase; pH was adjusted to 3.0 with orthophosphoric acid. Detection was carried out using UV detector at 238 nm. The method was carried out on a Shim-Pack CLC, ODS (C-18), 5 µ column and dimension of column was 250×4.6mm. The retention time of nifedipine was 3.401 min. The developed method was validated in terms of linearity, precision, accuracy, precision, limit of detection and limit of quantitation. The method was found to be linear in the range of 5-40 µg/ml. In the linearity study, regression equation and coefficient of correlation was found to be y = 361627x + 338075, r²= 0.9996.