Antibiotic resistance pattern in urinary isolates of Escherichia coli with special reference to extended spectrum β-Lactamases production

Abstract

Escherichia coli (E.coli) species are able to produce extended-spectrum β-Lactamase (ESBLs) that cause high resistance to the beta-lactam antibiotics. Present study was undertaken to know the occurrence of ESBLs productions in E.coli and to determining the antibiotic susceptibility patterns in resistant organisms for suitable therapeutic approaches. Clinical specimens of urine were collected from patients attending the Medical Microbiology Laboratories at Surat. Urine culture was done by conventional microbiological techniques. Isolation and identification of E. coli was carried out by standard procedure. Antibiotic sensitivity was done by the Kirby Bauer method. Production of ESBLs was detected by phenotypic confirmatory tests. During the study period 1500 urine samples were processed. Totally, 125 clinical isolates of E.coli were isolated and identified. ESBLs production was seen in 66% of isolates. All the ESBLs producing isolates were multidrug resistant (drug resistant to≥3 drugs). This study reveals existence of high percentage ESBLs producing E .coli as well as MDR E.coli. Hence, constant revision of antibiotic polices with infection control interventions are necessary for interruption of the transmission of these endemic isolates