Rapid clonal propagation of an endangered medicinal plant Plumbago zeylanica Linn.
Keywords:
In vitro, micropropagation, Plumbago zeylanica, medicinal plant.Abstract
The Present investigation has given a method for in vitro micropropagation of a valued endangered medicinal plant Plumbago zeylanica Linn. (Plumbaginaceae) called chitrak. In this study we used nodal part of P .zeylanica as explant. Explants were cultured on MS media (Murashige and Skoog) supplemented with different concentrations of Growth hormones like BAP (0.2-1.0mg/l) NAA (0.2-1.0mg/l) BAP+NAA (0.2-1.0mg/l) . The excellent shoot formation followed by 1mg/l BAP+ 1mg/l NAA in this combination was observed as 100% and maximum shoot length was recorded (5.38±0.99 cm.). Regenerated shoots were rooted on half strength MS medium supplemented with 1 IBA (0.2-1.0) mg/l and IAA (0.2-1.0) mg/l. But the maximum number roots (7.00±0.98) were produced when the medium supplemented with IAA similarly maximum root length (6.99±0.89cm) contributed by IBA at 1.0 mg/l on MS medium.
