Rapid In vitro propagation of medicinally important plant Solanum surattense Burm F.

Abstract

An efficient and reproducible protocol was established via organogenesis from nodal explants of Solanum surattense. The nodes are incubated and generated many buds on Murashige and Skoog (MS) medium supplemented with different concentrations of 6-benzylaminopurine 4.44-17.76 µM/l (BAP) and naphthaleneacetic acid 2.69 µM/l (NAA) in the 16 days. The highest rate of multiple shoot induction was observed on BAP (8.88 µM) and in combination with NAA (2.69 µM/l). The well-developed Plantlets were rooted on MS medium after being immersed in 4.90 µM/l of IBA for 24 h, 98.25% of the roots grow up two weeks later. After 20 days rooted plantlets that survived acclimatization grow well in the pots.