In-vitro experimental studies of selected biopesticides & their effect on selected plant pathogens

Abstract

The present work will focus on the importance of plant base biopreparations and their antifungal and antibacterial activity. The plants were selected for preparation of Extracts (biopreparations) to test their broad spectrum of resistance against for the selected plant pathogens as per the literature sources (Anonymous, 1948-76, Dhar., M.L., M.M.Dhar, B.N.Dhawan, B.N.Mehrotra and C.Ray. 1968, Chopra. R. N., Nayar. S. L. and Chopra. I. C. 1986, Bharath Kumar., R.2000.) There are about 15 indigenous plants were selected which are having diversified uses like medicinal, economic, timber and fibre yielding species like , Ocimum sanctum, Azadarichta indica, Thespesia populnea, Sida cordifolia, Pisonea alba, Nerium indicum, Ficus religiosa, Ricinus communis, Colotropis gigantea, Eucalyptus globulous Achyranthes aspera, Catharanthus roseus, Albizia lebbeck, Typha angustifolia and Agave americana. viz.of Guntur region (Vadlamudi, Tenali etc.). The Antifungal activity was tested against 2 pathogens, which are very much prone  to cause severe damage to the commercial crops viz., Solanum melongena and Gossypium herbacium and the anti bacterial activity was tested against the 2 pathogens, which causes considerable amount of  yield loss against the crops like Lycorpersicum esculentum and Citrus limonium. A total number of 90 plant extracts were prepared as an individual of 45 ethanol, methanol and acetone solvent extracts belongs to the 15 individual plant species (plant parts –leaf, St.b etc.) and another set of 45 plant extracts were prepared in combination  ethanol, methanol and acetone solvents. All these plant extracts were subjected against their antibacterial and anti fungal screening analysis, out of these 90 plant extracts (both individual and in combination) 44 plant extracts have been expressed the cognizable zone of expression i.e. 25 mm > 30 mm inhibition. Therefore these combinations were suggested for further analysis of producing a novel broad spectrum of biopesticides for crop protection.