Evaluation of Culture Medium for the Growth and sporulation of Rhizoctonia bataticola Causing Dry Root Rot of Chickpea (Cicer arietinum)

Abstract

Rhizoctonia bataticola (Taub.) is the cause of dry root rot of chickpea. This Pycnidia phase is found in Macrophomina phaseolina (Tassi) Goidis. It is a fungal pathogen that lives in the soil. This research was conducted at Department of Plant Pathology, SVPUA&T Meerut. In this research, dry root rot affected samples were collected from Chickpea fields. Ten different culture media were used in this study for evaluation for the best growth of Rhizoctonia bataticola. The fungus R. bataticola gave maximum mycelial growth on potato dextrose agar (87.93 mm) followed by cornmeal agar 80.16 mm and oatmeal agar 75.26 mm, while potato dextrose Bengal agar showed the least growth on solidmedium at 30.21 mm. Of the ten liquid broths tested, potato dextroe broth had the highest mean mycelial dry weight (154.20 mg), followed by cornmeal broth (125.80 mg), oatmeal broth (123.30 mg), and V-8 juice broth (117.40 mg). . Potato dextrose (53.66 mg) gives the least mycelial dry mass in Bengal broth. Sclerotia production was noticed highest in PDA and lowest in SDA.