Isolation of seed borne mycoflora of chickpea and its in vitro evaluation by some known bioagents

Abstract

Due to hazardous effect of chemical fungicides, search for safer alternative to control the pathogen is better choice. This led to trials on the use of bioagents to control the pathogen. In the present study in vitro antagonistic activity using the dual culture technique was undertaken to assess the potential of six Trichoderma species .It showed inhibitory effects on some common pathogens of chickpea namely Aspergillus flavus, Aspergillus fumigatus. The test antagonists grow faster than the pathogen and produced inhibition zones thereby limiting the growth of the pathogen. Maximum growth inhibition of A. flavus (74.53%), A. fumigatus (77.77%) were found with the use of Trichoderma harzianum (T2) in dual culture. In in vitro tests, all Trichoderma isolates showed significant differences in inhibiting the colony growth of the fungal pathogens.