HPLC Estimation of Khellin in Ammi majus L. Extracts and Fumaric acid in Fumaria parviflora L. Extracts

Abstract

This study presents the quantitative analysis of two important phytocompounds: Khellin from Ammi majus L. and Fumaric acid from Fumaria parviflora L. using High-Performance Liquid Chromatography (HPLC). The analysis was conducted by optimizing chromatographic conditions, developing calibration curves, and applying these to determine the concentration of these bioactive compounds in the plant extracts. The results show that Ammi majus contains 6.01% Khellin, and Fumaria parviflora contains significant levels of Fumaric acid. This analytical approach highlights the precision and applicability of HPLC in herbal phytocompound analysis.