FT-IR, FT-Raman and GC-MS analyses of biochemical compounds in Ophrys apifera Huds (Orchidaceae) species

Abstract

Fourier-transform infrared spectroscopy (FT-IR), fourier transform
raman spectroscopy (FT-Raman) and gas chromatography-mass
spectrometry (GC-MS) techniques were employed for biochemical
characterization of acetonic, methanolic and ethanolic flowers of Ophrys
apifera Huds species extracts, as a new Ophrys species in Syria. FT-IR
spectra 1100 cm-1 peak assigned to C–O secondary alcohol stretch C–O
stretch (Ethers) and 3000 cm-1 peak assigned =C-H stretch (aromatics)
groups were mainly detected as a common peaks with the three
examined extracts. As for FT-Raman spectra, 1500 cm-1 peak assigned to
C=C stretch aromatic (aromatics) group was mainly detected as a
common peak with the three examined extracts. Whereas, in GC-MS
assay, 9-Octadecenamide (Z)- compound (oleamide) was mainly
presented as a major and common compound 67.76%, 85.87% and
87.49% with acetonic, methanolic and ethanolic extracts, respectively.
All the above mentioned components exhibited a potential role in
pharmaceutical and medicine researches and applications. Thereby, more
attention should be given to these components to be handled in the future
work.