Studies of Microbiological Analysis and Identification of Microorganisms in High TDS and Low TDS Waste Water of CETP Naroda

Abstract

Industrial wastewater from chemical industries contains a wide range of pollutants and microorganisms which can have adverse effects on the environment and public health. This study investigates the microbial and physicochemical characteristics of wastewater collected from different treatment stages of Common Effluent Treatment Plant (CETP), Naroda, Ahmedabad. Parameters like pH, Biochemical Oxygen Demand (BOD), Chemical Oxygen Demand (COD), Total Dissolved Solids (TDS), Total Suspended Solids (TSS), Chloride and Ammoniacal Nitrogen were analysed using standard methods. The presence of microorganisms in the raw wastewater sample despite the TDS concentration (25,000 to 15000ppm) indicates that they can decompose organic matter present in the polluted water and their identification was analysed using various agar media.

Microbiological analysis was performed using culture-based techniques on selective and differential media including Nutrient Agar, McConkie Agar, EMB Agar, XLD Agar, PDA, Rose Bengal Agar, Robertson’s Cooked Meat Medium and high-TDS media. The results revealed heterotrophic bacteria such as Pseudomonas and Bacillus species, indicator organisms such as Escherichia coli, pathogenic Salmonella species, halophilic bacterial and fungal isolates mainly belonging to Mucor species. These findings shed light on the microbial diversity of wastewater entering CETP.