Antibacterial Effects of Some Antibiotics and Essential Oils Against Brucella abortus Inside Goat’s Macrophages and New Promising Treatments

Abstract

Brucellosis is an infectious disease caused by bacteria. Animals that are most commonly infected include sheep, cattle, goats, pigs, and dogs. Nowadays, the resistance of microbial infections to antibiotics has threatened the health of societies. Thus, this article introduces the antibacterial effects of some antibiotics and plant-derived essential oils so that they can be used as promising choices to develop a new anti-Brucella therapy. B. abortus isolate was obtained from milk samples collected in previous work from different Syrian provinces. Essential oils extraction was acquired using water steam distillation device. Macrophages were isolated from blood and infected with B. abortus at a ratio of 1:100 bacteria/macrophage.

Brucella strains have been shown resistant to most antibiotic groups used in this study, whereas it was detected an excellent synergistic activity between Levofloxacin- Cefprozil, and Levofloxacin – Tetracycline with a log₁₀ decreasing from 3.367, 3.303 to a value of 0 after 96h of infection respectively.

Cinnamon 1% was revealed the best antibacterial activity against B. abortus strains with a decreasing value of log₁₀ reached 0.95 after 96h of infection. A strong significant inhibitory effect was observed for Cinnamon 0.1% in combination with a 1% concentration of the other plant's oil extracts.