In Vitro Antibacterial activity of Hygrocybe parvula (Peck) Pegler

Abstract

The antibacterial activities of petroleum ether, chloroform and methanol extract of mushroom H. parvula was analyzed in vitro by agar well diffusion method. The yields of extracts obtained from different solvents were petroleum ether (10.88) gm, chloroform (11.21gm) and methanol (51.16gm). The growth inhibitory effects of crude extracts were tested against three plants and six human pathogenic bacteria. The maximum antibacterial activity of petroleum ether extracts of H. parvula was found against A. tumefaciens (16mm) at 100% concentration and minimum against X. campestris (3mm) at lower concentration of chloroform and methanol extract in plant pathogenic bacteria. Whereas petroleum ether extracts were showed more inhibition zone against P. aeuroginosa (18mm), at 100% concentration in human pathogenic bacteria, the activity was less than the standard Tetracycline and Ciprofloxacin. The extract shows increasing inhibitory activity with increase in concentration (12.5% -100%). The antibacterial activity of all the solvent extracts were calculated by measuring inhibition zone formed around the well.