VDR Taq1 gene polymorphism in osteoporosis: A study from central India

Abstract

Menopause is associated with an imbalance in bone metabolism, and the first five to ten years after menopause is the
period of higher bone turnover and bone loss Approximately 35% of postmenopausal women lose significant
amounts of bone mineral during this period and are at a higher risk for osteoporosis and fragility fractures later in
life Introduction. Osteoporosis is a polygenic disorder that is determined by the effects of several genes, each with
relatively modest effects on bone mass and other determinants of fracture risk. In this study, we investigated the
association between the Taq 1 polymorphism of vitamin D receptor (VDR) gene and BMD in a population of
vindhayan region. Taq 1 polymorphism was analyazed by PCR –RFLP METHOD. PCR Amplification products of
293bp, 340bp and 47bp were obtained. product which was digested with taq-1 enzyme . TT homozygous for the
absence of TaqI site 340 bp only; tt homozygous for the presence of TaqI site 293 bp and 47 bp, in case of
heterozygosity Tt, all three bands (340 bp, 293 bp and 47 bp) were exhibited. The pattern of genotype and allele
distribution in disease and HC group suggested strong association of VDR Taq1 polymorphism in postmenopausal
osteoporosis susceptibility.