Study of the Physiological Alterations Induced by Cisplatin in Miceandthe Possible Protective Role of Green Tea

Abstract

Cisplatin (CDDP) isan anti-cancerDNA alkylating chemotherapeutic agent act against a variety of
tumors.The present study aimed to evaluate the possible protective effects of green tea on the physiological
parameters in mice chronically treated with CDDP. Four groups of mice were examined: a control mice
saline PBS solution (group I), mice treated with CDDP (group II), mice treated with CDDP and green tea
(group III), and normal mice treated with green tea (group IV). All animals were treated for successively
five days and killed one week after the last treatment. The results recorded that CDDP treatment significantly
decreasedthe levels of white blood cells (WBCs), red blood cell distribution width (RDW) and lymphocytes
count. Also, CDDP increased the hepatocytes oxidative stress which characterized by increasing prooxidants
xanthine oxidase (XO), thiobarbituric acid-reactive substances (TBARS), and decreasing of antioxidants
glutathione peroxidase (GPx). As a result, hepatocytes injury took place that characterized with serum
aspartate aminotransferase (AST), alanine aminotransferase (ALT) andalkaline phosphatase (ALP) activities.
The treatment of mice with green tea to CDDP group (group III) or mice treated with green tea alone group
(group IV) successfully normalized the physiological parameters in form returning WBCs, RDW and
lymphocytes counts to normal levels, and decreased the hepatocytes oxidative stress which characterized by
decreasing Prooxidants (OX, TBARS) and increasing of antioxidants GPx reflected by significant decrease
in the serum activities of AST, ALTand(ALP) activities.