Evaluation of Antioxidant Potential through DPPH and ABTS Assays: A Study on Polyherbal Formulation

Abstract

The study investigates the antioxidant potential of a polyherbal formulation (PHF) using DPPH radical scavenging, ABTS radical scavenging assays, and total phenolic content (TPC) estimation. The DPPH assay revealed that the hot water extract of PHF showed a maximum scavenging activity of 83.6%, comparable to the standard Gallic acid (86.6%), while the ethanolic extract exhibited 54.5% activity. ABTS assay demonstrated higher antioxidant activity in the ethanolic extract (86.5%) compared to the hot water extract (82.3%). The IC50 values confirmed the superior radical scavenging capacity of the ethanolic extract for ABTS and hot water extract for DPPH. TPC analysis revealed 72.6 mg/g of phenolic content in the hot water extract, significantly higher than the ethanolic extract (31.22 mg/g). The findings highlight the potential use of PHF hot water extract as an effective natural antioxidant alternative.