Formulation of Polyherbal cream and evaluation of their antimicrobial potential

Abstract

In recent years, there has been a progressive gradual development in the use of medicinal plants in developing nations, owing to the fact those herbal medicines are safe and have few side effects, especially when compared to synthetic pharmaceuticals. As the occurrence of unwanted side effects appears to be less common with herbal medications compared to allopathic treatments, the usage of herbal drugs is increasing, resulting in a rapid increase in the number of herbal drug makers. Since they are typically less expensive than synthetic medications People have been increasingly using herbal medicines during the last few decades. The present study was commenced to evaluate the quantitative profiling and antimicrobial activity of cream prepared from extracts of traditional plants like Kanthkari, Rasana, and Nirgundi. In the present study, a cream was formulated which comprised of an extract of plant materials. The dried plant materials of Kanthkari, Rasana, and Nirgundi were individually extracted using the maceration method. These three different types of extracts were used for the preparation of cream and evaluated for the HPTLC profiling and antimicrobial activity. The HPTLC profiling of the extract and formulation was performed on pre-coated silica gel Aluminum Plate 60F-254 using Toluene: Ethylacetate: FormicAcid (8:1.5:0.5v/v/v) as mobile phase and a further quantity of Quercetin was evaluated in extract and formulation. The antimicrobial activity of prepared cream was evaluated against Escherichia coli and Staphylococcus aureus by disk diffusion method using reference disk of antibiotics. The result of antimicrobial the activity was determined by comparing the results of the zone of inhibition of formulation and solvent extract. The formulation showed significant activity against the tested bacterial pathogens. The disk diffusion method was used to assess the antibacterial activity of methanolic extract using a reference disk of antibiotics. The Rf value and spectral scanning of standard Quercetin (Rf 0.22) match with the values of extract and formulation indicate the presence of Quercetin in extract and formulation. Considering the ability of the golden treasure present in Kanthkari, Rasna, and Nirgundi. The Quality Control profiling of formulation and extract was performed by HPTLC, and antimicrobial activity was established by disk diffusion method. Formulated cream can use as a potential antibacterial against various bacterial infections.