Para-phenylenediamine toxicity in Henna product in Libya

Abstract

Henna is very famous inside the Libya; it's a part of the style of life and traditions. Allergy to natural henna isn't
familiar; however the addition of para-phenylenediamine (PPD) to the natural henna increases the chance of allergic
touch dermatitis. The objectives of the study were to identify the presence and determine the concentration of PPD
in henna available in Libya and identity of heavy metals in these samples. To determine quantitatively and
qualitatively presence of PPD in samples of henna we used High Performance Liquid Chromatography (HPLC) and
nine elements (Pb, Hg, Al, Cr, Mn, Co, Ni, Mo and Ag) were quantified subsequently by Mass Spectrometry.
Natural henna and jawee el henna and henna mix with unknown addition components were determined by a by
GC/MS. The study showed that PPD was present in jawee el henna sample at concentration 100% and henna mix
with unknown addition samples at concentrations ranging between11.01% and 24.66, At the same time as all of the
black henna samples at concentrations ranging among 9.74% and 16.34% which is higher than that red henna. In
some studied metal as Aluminum, Lead, and nickel mean concentration of red henna samples was lower than that
black henna.