Evaluation effects of hydrogen peroxide on health status and quality characterization of Nile tilapia (Oreochromis niloticus)

Abstract

This study was based on investigation effects of hydrogen peroxide on health status, physicochemical,
microbiological, Sensorial and nutritional quality for two stocking rates for intensive culture of tilapia mono sex
males (10 and 20 fish /m3
). In which, three doses of hydrogen peroxide H2O2 were used; (0, 350 and 500mg/m3
) for
24 weeks to both stocks. Chemical, microbiological and sensory analyses were performed to investigate quality
changes, and to determine the best sensorial and nutritional quality. The proximate, peroxide values (PV),
thiobarbituric acid values (TBA), total bacterial count (TBC) and mould count were found statistically significant (P
≤ 0.05), in the low density stocked fish treated by 350 mg H2O2. Finally, (after 24 weeks) 10 fish /m3 recorded the
best of nutritional quality and health to ensure safety by avoiding any threat to the health of the consumer. Protein%,
fat% and ash% contents of low stocked density fish treated by 350 mg H2O2 samples had marked increase compared
to the other treatment. The result indicated a much lower content of PV, TBA and TVB-N values in the low
density stocked fish treated by 350 mg H2O2 in comparison with the other treated fresh fish samples other than
control. These PV, TBA and TVB-N values were much lower than acceptable limit. The result also revealed that
samples of low density stocked fish treated by 350mg H2O2 had lower TBC and mould count than the control. The
organoleptic results showed that samples of low density stocked fish treated by 350mg H2O2 had the best
acceptance, overall acceptability and were significantly different (P ≤ 0.05), when compared to the control. Based
on results obtained in this study, it could be concluded that, levels of intensive 10 fish /m3 were better than 20 fish
/m3
. In addition to that, fish treated by 350 mg H2O2 gave the best nutritional quality and health. While stocking 20
fish /m3 treated with hydrogen peroxide was of best economic efficiency when compared to other treatments.