In-Vitro Evaluation of Ethanolic Extract of Convolvulus pluricaulis Choisy for Anti-Inflammation & Memory Enhancing Activity

Abstract

The aim of the present investigation is to evaluate In-Vitro Evaluation of Ethanolic Extract of Convolvulus pluricaulis Choisy for Anti-Inflammation & Memory Enhancing Activity. Whole plant materials were dried under shade and subjected to coarse powder for extraction process. Accurately weighed quantity of whole plant material was extracted using 95 % ethanol by soxhlet apparatus for 72 h. Qualitative chemical tests of ethanolic extracts were subjected to various chemical tests to detect various phytoconstituents. The human red blood cells (HRBC) membrane stabilization had been used as a method to study anti-inflammatory activity. In this method, blood was collected from healthy human volunteers who had not been taken anti-inflammatory drug for 2 weeks prior to the experiments. AChE activity was measured using a modified 96-well microplate assay (14) based on Ellman’s method (15). The enzyme hydrolyses the substrate acetylthiocholine resulting in the product thiocholine which reacts with Ellman’s reagent (DTNB) to produce 2-nitrobenzoate-5-mercaptothiocholine and 5-thio-2-nitrobenzoate which can be detected at 412 nm. The preliminary phytochemical analysis revealed that different active constituent present in different extracts such as carbohydrates, proteins, amino acids, fat, oils, steroids, terpenoids, glycosides, alkaloids, tannins and other phenolics compounds. At a concentration of 500 μg/ml, the extract produced 72.60% protection of RBC haemolysis as compared with 73.84% produced by prednisolone. The ethanolic extract of selected plant showed 40.22% inhibition. The Diclofenac sodium showed 48.50% inhibition against denaturation of protein. The ethanolic extract of selected plant materials showed 42.65% inhibition respectively. The Diclofenac sodium showed 63.89% inhibition against proteinase inhibitory activity. In conclusion, it can be stated that the ethanolic extract has beneficial effects in long lasting in membrane stabilizing method, inhibition of protein denaturation method and proteinase model.