Phytochemical and HPTLC studies of methanolic extract of Indigofera tinctoria (Fabaceae)

Abstract

The aerial parts (fresh leaves) of the plant Indigofera tinctoria were collected powdered and the powder was initially defatted with petroleum ether (60-80°C). The marc was further macerated with methanol for 72 hrs. The extract was preserved at room temperature for further studies.Preliminary Phytochemical screening was done which revealed the presence of sugars, tannins, polyphenols, alkaloids, aminoacids, triterpenoids, saponins, flavoniods.The HPTLC techniques were used for qualitative determination of possible number of components from the methanolic extract . A solvent system was optimized in order to get maximum separation on plate and presence of various phytochemicals present was confirmed by the use of different spraying reagents.