Stability indicating LC estimation of ramipril and hydrochlorthiazide in its bulk and tablet formulation
Keywords:
Ramipril, Hydrochlorothiazide, High Performance Liquid Chromatography, Limit of Quantification, Limit of Detection.Abstract
A simple, rapid and sensitive HPLC method has been developed for the simultaneous determination of Ramipril and Hydrochlorothiazide in their dosage forms. Acetonitrile: Phosphate buffer (0.01 M) adjusted to pH 2.6 with O-phosphoric acid, was used as the mobile phase. A CHROMO Sil C-8 (4.6*250 mm) column was utilized as stationary phase. Detection was affected spectrophotometrically at 210 nm. The method was also applied for the determination of Ramipril in the presence of its degradation products. Linearity ranges for Ramipril and Hydrochlorothiazide were 10-50 and 10-150 mg/ml, respectively. The proposed method was further applied to the analysis of tablets containing the two drugs, the percentage recoveries S.D. (n_5) were 99.66% and, 99.63% for Ramipril and Hydrochlorothiazide respectively.
