Pharmacognostical and physicochemical analysis of pathyadi varti – A polyherbal ayurvedic formulation

Abstract

Many of the herbal formulations indicated for internal use in various ailments, are very useful in eye diseases also. Classics have also described some formulations as local application in the form of vartis (Pills), anjana (Collyrium), and eye drops etc. These are very effective but most of them still to be standardized as far as their quality, S.O.P., properties and characteristics. In the present study a systematic approach has been evolved and effort has been made to develop well designed methodology for the standardization of Pathyadi varti - an Ayurvedic formulation for local application. The finished product was subjected to organoleptic, microscopic characterization, physico-chemical screening, phyto-chemical analysis and HPTLC studies. The pharmacognostical evaluation shows fragments of mesocarp cells, sclereids, tannin contents of Haritaki; pitted vessels, lignified fibres, and larger starch grains of Yashtimadhu; beaker shaped stone cells and oil globules from Maricha. The absence of prysmatic crystals was significant outcome. The Phytochemical analysis shows the presence of alkaloids, tannins, flavonoids, saponins and anthraquinon glycosides. Spots obtained in HPTLC were found resembling spots of glycyrrhizin at R_f  0.27 and piperine at R_f 0.60 as reported in previous studies.