Isolation, molecular characterisation and sequencing of cholesterol degrading bacteria

Abstract

Bacillus cereus from soil of agriculture waste using cholesterol-Tween-80 medium. Conformation of cholesterol degrading activity by using halo formation and estimation technique. Optimum growth was observed on 37°C, 7.0 pH on cholesterol-Tween-80 medium. Morphology characterization and molecular characterization (i.e.) 16s rRNA sequencing was performed to identify the strain. The cholesterol oxidase enzyme was purified and electrophoresised.This study was concluded that the isolate was confo rmed cholesterol degrading bacteria and produce the intermediate product 4-Cholesten-3-one by the enzyme cholesterol oxidase, it was proved by estimation and Halo formation. This Bacillus cereus was degrades cholesterol and takes as a carbon source for its growth.Cholesterol Oxidation occurs in both Oxic pathway and Anoxic pathway. But the Oxic pathway is the best for cholesterol oxidation. Cholesterol oxidase enzyme oxidize the cholesterol to 4-cholesten-3-one with the reduction of O₂ to H₂O.It is the first step in cholesterol degradation. The complete degrading pathway in cholesterol degradation has limited research. Thus, more molecular systematic studies need to be undertaken in this direction.