Effect of 24-Epibrassinolide induced changes in Seed germination, Growth and Biochemical Composition in Early seedling stages of Brassica juncea (L.) Czernj

Abstract

Brassinosteroid (BR) is the endogenous plant growth regulator involved in various physiological processes of plant growth and development. In the present experiment, an attempt has been made to understand the 24-epiBrassinolide (eBR) induced responses in Brassica juncea. Application of eBR at 1µM and1.5μM significantly enhanced the rate of Brassica seed germination to a level of 92% and 94% respectively in Brassica.  Dark incubated Brassica seedlings behaved differently to the application of BR. The internode length rather than root length was significantly increased upon eBR treatment. Among the concentrations, only 1.0 µM and 1.5 µM were found to be useful in triggering the growth responses. With increase in time, growth and biochemical parameters such as soluble protein, glucose content and β- amylase activity were also increased under BR treatment. Thus the exogenous application of BR proved to be physiologically and biochemically efficient in improving the vegetative growth of Brassica.