Genetic diversity of Ralstonia solanacearum causing bacterial wilt of brinjal

Abstract

In the present study, isolates were identified as R. solanacearum on the basis of phenotypic characters as well as
based on PCR amplification. All the virulent R. solanacearum from Occurrence of bacterial wilt in solanaceous
crops were noted in 8 states of India as: (ICAR-Res. complex NEH Region). morphologically resembled those from
other regions of the world (Williamson et al., 2002) All isolates of R. solanacearum were collected isolated from
Himachal Pradesh (1 isolates), Jharkhand (4 isolates), Uttarakhand (8 isolates), Odisha (9 isolates), IIHR Bangalore
(3 isolates), Shillong (2 isolates), Karnataka (3 isolates) and West Bengal (11 isolates). And producing typical white
coloured fluidal colonies with pink centres and irregular in shape (Hayward, 1964). The disease affects brinjal crops.
Maximum incidence of disease was found in rainy season from July to October up to 60% where as in summer
season April to June up to 10%., however, the disease incidence was found up to 60% in Solan districts in Himachal
Pradesh and Nainital (mostly foothills area, Golapar) in Uttarakhand. Brinjal crop was moderately infected by R.
solanacearum and disease incidence was varied 15 - 40% in Palampur area in Himachal Pradesh, 4- 15 % in Plandu
district of Ranchi area in Jharkhand. It has been reported Characterization of bacteria degrading 3-hydroxy palmitic
acid metylester (3OH-PAME), a quorum sensing molecule of Ralstonia solanacerum Letters.And It was observed
that the Studies on Pseudomonas solanacearum (E.F. Smith) causing wilt of brinjal, in Mysore state.