Development and Validation of RP-HPLC method for Simultaneous Estimation of Cefexime and Linezolid

Abstract

This work is concerned with application of simple, accurate, precise and highly selective reverse phase high performance liquid chromatographic (RP-HPLC) method for simultaneous estimation of cefexime and linezolid in combined dosage form. Chromatographi separation was achieved by using a reverse phase C18 coloum (Hypersil BDS 250X 4.6X5µm). The mobile phase composed methanol: potassium dihydrogen phosphate buffer in ratio 40:60 at flow rate of 1ml/min .The pH was adjusted to 3.5 with TEA. Detection was carried out using a UV-vis detector at 277 nm. The mean retention time of cefexime and linezolid was found to be 3.76 min 6.55 min respectively. The method was found to be linear in the range of 5 - 15 µg /ml and 15 -45 µg /ml for cefexime and linezolid respectively, with mean recovery of 99.81-100.14 % cefexime and 99.99-100.47 % for linezolid the correlation coefficient for both cefexime and linezolid were closed to one. The developed method was validated according to ICH guidelines and values of accuracy ,precision and other statistical analysis were found to be in good accordance with the prescribe values thus proposed method was successfully applied for simultaneous determination of cefexime and linezolid in routine analysis of formulation.